John Stones went off in the 37th minute against Germany
The Manchester City defender appeared to injure his hamstring and was replaced in the 37th minute by club team-mate Kyle Walker.
Walker, who has just returned from a muscle injury, started England's 1-0 defeat to Italy on Friday.
City host neighbours Manchester United in the Premier League on Sunday, 14:00 BST.
Both Stones and Walker have seen the start to their season disrupted by injury.
Stones missed City's Champions League victory at Sevilla while right-back Walker suffered a problem in the Premier League draw at Aston Villa at the start of September.
-- Courtesy of BBC Sport